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KMID : 0617319980070010128
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Journal of Pharmacetical Sceiences Ewha Womans University
1998 Volume.7 No. 1 p.128 ~ p.132
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Neuraminidase Treatment Enhances Allogeneic Stimulation of Unprimed CD8^+ T Cells
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Kim, Kilhyoun/College of Pharmacy, Ewha Womans University
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Abstract
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Many cell types are know to stimulate CD8^＋ T cells in allogeneic recognition such as mixed lymphocyte reaction (MLR). Whereas dendritic cells are most potent among them, T cells are usually considered very poor in stimulating CD8^＋ T cells although there are some tumor cells that are weakly stimulatory. T cells, as a stimulator, cultured in the presence of concanavalin A that were otherwise nonstimulatory to CD8^＋ T cells appeared to stimulate CD8^＋ T cells strongly when they were pretreated with neuraminidase. The enhancement of MLR by neuraminidase could be achieved by treating either the stimulators or responders with neuraminidase. Removal of negatively-charged sialic acid moieties from the cell surface, which reduced electrostatic repulsion between responders and stimulators to give better cell-cell contact might be responsible for the enhanced MLR In addition, neuraminidase treatment also appeared to deliver activation signal to responding T cells since it could activate CD8^＋ T cells in synergy with phorbol myristate acetate. The maximal responses were observed when both responders and stimulators were treated with neuraminidase.
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